Contact an Account ManagerDetermination of 3-amino-1,2,4-triazole (amitrole) in tissues with electrochemical detection by ion pair HPLC
Summary
Type: HPLC
Application #: 108710
Catalog#: 720014.40 - MN HPLC Column, Nucleosil C18, 100A, 5um, 4x250mm
Detection: electrochemical, glassy carbon working electrode at +1.2 V versus Ag/AgCl
Conditions: eluent: acetonitrile - water (30:70) containing 0.002M dodecylsulphate, 1g/l lithium perchlorate trihydrate, adjusted to pH 3.2 with 6% perchloric acid
flow rate 0.75ml/min
pressure 165bar
Authors: Ternes, W. et al.
Source: Z. Anal. Chem. 326 (1987) 757–759
Details
Legend: Fig. 5. Typical chromatograms of amitrole (A) in liver, a) blank, b) 75ng
amitrole/g liver added, IS = internal standard.